Re: calibration in photomicroscopy
Re: calibration in photomicroscopy
- Subject: Re: calibration in photomicroscopy
- From: Roger Breton <email@hidden>
- Date: Thu, 27 Jan 2005 12:42:11 -0500
> Hello,
> I¹m currently working to get a digital camera system for
> photomicroscopy set up in my microbiology lab. Because we¹re an FDA/ISO
> regulated lab, we need to be able to show that the camera is working correctly
> and has been calibrated on a regular schedule. The system we¹re looking at
> getting uses ICC profiles to ensure color from slide to screen, so generating
> an ICC profile then comparing it to the manufactures profile, or something
> like an IT8.7 target for microscopes, would work for that. I know it¹s
> possible to get deltaE for a particular color with some profiling software,
> but can I generate something like a deltaE for the whole target? Is there
> software on the market for Windows that already does this, or can a sampling
> of some colors be entered into an excel sheet and an average taken? I¹d
> appreciate any ideas people have on the subject.
>
> Thank you,
> Kurt Fleschner
> Biotest Labs, Inc.
One way you could try is see if you could take pictures of 24 Munsell color
sheets corresponding to the ColorChecker II 24 patchs chart. Once you know
what RGB readings your camera is returning on each patches photographed
individually, you could "paste" them in into a digital capture of a whole
ColorChecker II chart, in Photoshop, and submit that for profiling. I'm sure
it would work.
Regards,
Roger Breton | Laval, Canada | email@hidden
http://pages.infinit.net/graxx
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