Re: calibration in photomicroscopy
Re: calibration in photomicroscopy
Hi Kurt -
There are several considerations. In concept, you could shoot a target with a traditional lens and create a profile with basICColor dCam. When mounting the camera to a scope, one would need to take into account the neutrality of the apochromatic lens/coatings and allow consideration for fluorescein or polarized images. There is no truly scientific approach to these accommodations. Getting the CCD profile right will put you within the same range as traditional transparency, however.
- Jon Meyer (RBP)
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On Jan 27, 2005, at 12:35 PM, Kurt Fleschner wrote:
<x-tad-bigger> I’m currently working to get a digital camera system for photomicroscopy set up in my microbiology lab. Because we’re an FDA/ISO regulated lab, we need to be able to show that the camera is working correctly and has been calibrated on a regular schedule. The system we’re looking at getting uses ICC profiles to ensure color from slide to screen, so generating an ICC profile then comparing it to the manufactures profile, or something like an IT8.7 target for microscopes, would work for that. I know it’s possible to get deltaE for a particular color with some profiling software, but can I generate something like a deltaE for the whole target? Is there software on the market for Windows that already does this, or can a sampling of some colors be entered into an excel sheet and an average taken? I’d appreciate any ideas people have on the subject.</x-tad-bigger>
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